Thermo Scientific™ Phusion Hot Start High-Fidelity DNA Polymerase

Increase the specificity of PCR amplification by preventing non-specific extension with this highly accurate DNA polymerase, now available with a hot start modification.

Brand: Thermo Scientific™

Manufacturer Part Number: F-540L

UNSPSC: 12352200

Code: 87

Additional Details:
Additional Details: Weight: 1.71460kg

Product Code. 10004397

Quantity Price
1 £ 487.0 / 500 units
EU Stock 18
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  • Description and Specification
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Description and Specification


Storage Requirements Recommended storage temperature -20°C. Stable for one year from the assay date.
Quantity 500U (2U/µL)
Description Phusion™ Hot Start High-Fidelity DNA Polymerase
Includes Phusion Hot Start High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration.
For Use With (Application) Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray

Storage buffer

20 mM Tris-HCl (pH 7.4 at 25°C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 200 µg/ml BSA and 50% glycerol.


Recommended storage temperature -20°C.

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