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Thermo Scientific™ Phusion™ Blood Direct PCR Kit

Perfrom PCR directly from whole blood without DNA purification.

Brand:  Thermo Scientific™ F547S

Code : 87

Additional Details : Weight : 1.71460kg

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Product Code. 10543488

  • £78.50 / 100 reactions


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Description

Description

Phusion Blood Direct PCR Kit is designed for amplification of DNA from whole blood without a prior purification step. The kit utilizes a modified Phusion Hot Start II High-Fidelity DNA Polymerase. This highly processive and robust PCR enzyme is resistant to the inhibitors present in blood and retains its activity even at 40% blood concentration. Phusion Blood Direct PCR Kit allows analysis of blood samples with different anticoagulants, storage conditions and from various species.
  • No need for DNA extraction
  • Allows up to 40% blood in the PCR reaction
  • Tolerates heparin, citrate, and EDTA, and is compatible with frozen blood or blood stored on various types of filter papers
  • Modified Phusion Hot Start II DNA Polymerase gives specific and accurate results with high yields and short protocol times

Includes:

Phusion Blood DNA Polymerase, 2x Phusion Blood PCR Buffer (includes dNTPs and MgCl2), EDTA, MgCl2, DMSO, universal control primer mix

Recommended for:

Detection of SNPs with PCR-RFLP. Genotyping.

Specifications

Specifications

amplification of DNA directly from whole blood.
Phusion™ Blood Direct PCR Kit
Store at -20°C.
Phusion Blood DNA Polymerase, 2x Phusion Blood PCR Buffer (includes dNTPs and MgCl2), EDTA, MgCl2, DMSO, universal control primer mix
100 x 20µL Rxns
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