Phospho-ZAP-70/SYK (Y319/Y352) Mouse anti-Human, Mouse, PerCP-eFluor 710, Clone: n3kobu5, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 46-9006-42

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15539386

Quantity Price
1 £ 219.5 / 100 tests
Estimated Shipment date
from Supplier 05-06-2017
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Description and Specification

Specification

Antigen Phospho-ZAP-70/SYK (Y319/Y352)
Applications Flow Cytometry (Intracellular Staining)
Clone n3kobu5
Concentration 5μL (0.03μg)/test
Conjugate PerCP-eFluor 710
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Zeta chain-associated protein of 70 kD/Spleen tyrosine kinase
Host Species Murine
Isotype IgG2b
Quantity 100 Tests
Regulatory Status RUO
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Target Species Human
Target Species Murine

This n3kobu5 monoclonal antibody recognizes human and mouse zeta chain-associated protein of 70 kD (also known as ZAP-70) and spleen tyrosine kinase (also known as SYK) when phosphorylated on Y319 and Y352, respectively. ZAP-70 and SYK are members of the SYK protein tyrosine kinase (PTK) family that are phosphorylated and activated by Src family PTKs. ZAP-70/SYK Y319/Y352 are located in the so-called interdomain of ZAP-70/SYK (between the N-terminal dual SH2 domains and the C-terminal kinase domain).

Phosphorylation of ZAP-70 Y319 by Lck is necessary for T cell receptor (TCR)-dependent association of ZAP-70 with Lck and phospholipase C gamma and subsequent activation of calcium-dependent and Ras signaling cascades. SYK Y352 phosphorylation by Fyn/Lyn is critical for propagation of B cell receptor (BCR) signaling and for B cell development.

Specificity of this n3kobu5 clone was determined by ELISA, flow cytometry, and western blotting.