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phospho-Tyrosine Mouse anti-Human, Mouse, eFluor® 660, Clone: pY20, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-5001-41

Code : NEW

Additional Details : Weight : 0.23750kg

Product Code. 15579966

  • £79.25 / 25 tests

Estimated Shipment
26-10-2020

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Description

Description

Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the Best Protocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
Specifications

Specifications

Phospho-Tyrosine
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
pTyr; pY; Phosphotyrosine
IgG2b, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
Human, Mouse
Flow Cytometry
pY20
eFluor™ 660
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
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For Research Use Only.