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Phospho-Syk (Tyr348) Mouse anti-Human, Mouse, APC, Clone: moch1ct, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-9014-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15568216

  • £85.00 / 25 tests

Estimated Shipment
06-11-2020

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Description

Description

Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the Best Protocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
Specifications

Specifications

Phospho-Syk (Tyr348)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P43405, P48025
Protein tyrosine kinase p72Syk
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
Flow Cytometry
moch1ct
APC
SYK
Liquid
SYK
IgG1, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
20963, 6850
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For Research Use Only.