missing translation for 'onlineSavingsMsg'
Learn More

Phospho-STAT6 (Tyr641) Mouse anti-Human, Mouse, PerCP-eFluor™ 710, Clone: CHI2S4N, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9013-41

Code : NEW

Additional Details : Weight : 0.23750kg

Product Code. 15589386

  • £91.50 / 25 tests

Estimated Shipment date
from Supplier 25-03-2020

Please to purchase this item. Need a web account? Register with us today!


Description

Description

All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. STAT6 protein is a transcription factor activated by cytokines, particularly interleukin-4 and IL13. (Stat6-/-) were found to be deficient in IL-4-mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 immune response. STAT6 is crucial for IL-4-mediated biological responses, and naturally in two isoforms: STAT6a and STAT6c.
Specifications

Specifications

Phospho-STAT6 (Tyr641)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P42226, P52633
Signal Transducers and Activators of Transcription 6, IL4 STAT, Interleukin 4 Induced
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
Flow Cytometry
CHI2S4N
PerCP-eFluor™ 710
STAT6
Liquid
STAT6
IgG2a, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
20852, 6778
Videos
Documents

For Research Use Only.