missing translation for 'onlineSavingsMsg'
Learn More

Phospho-STAT6 (Tyr641) Monoclonal Antibody (CHI2S4N), PE, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9013-42

169.34 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15527156

  • £254.00 / 100 tests
Estimated Shipment: 08-04-2024
to see stock.

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: This CHI2S4N monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 6 (STAT6) when phosphorylated on tyrosine 641. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to IL-4, STAT6 is phosphorylated on tyrosine 641 by JAK1 and JAK3. STAT6 signaling downstream of the IL-4 receptor promotes T cell growth and B cell production of IgE. Specificity of this CHI2S4N clone was determined by ELISA and flow cytometry. Applications Reported: This CHI2S4N antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This CHI2S4N antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used.

STAT6 protein is a transcription factor activated by cytokines, particularly interleukin-4 and IL13. (Stat6-/-) were found to be deficient in IL-4-mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 immune response. STAT6 is crucial for IL-4-mediated biological responses, and naturally in two isoforms: STAT6a and STAT6c.
Specifications

Specifications

Phospho-STAT6 (Tyr641)
Monoclonal
5 μL/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P42226, P52633
STAT6
Affinity chromatography
RUO
20852, 6778
4° C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
CHI2S4N
PE
STAT6
12S1644; D12S1644; IL4 STAT; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; signal transducer and transcription activator 6; STAT; STAT, interleukin4-induced; Stat6; STAT-6; STAT6B; STAT6C; transcription factor IL-4 STAT
Mouse
100 Tests
Primary
Human, Mouse
Antibody
IgG2a κ
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only.