missing translation for 'onlineSavingsMsg'
Learn More

Phospho-STAT3 (Tyr705), FITC, clone: LUVNKLA, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-9033-41

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15596606

  • £81.72 / 25 tests

EU Stock 3
Add to basket



Description: This LUVNKLA monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 3 (STAT3) when phosphorylated on tyrosine 705 (Y705). The STAT family represents seven transcription factors (STATs 1, 2, 3, 4, 5A, 5B, and 6) that are involved in many cellular processes including apoptosis, cell differentiation, and proliferation in a cell type- and cytokine-specific manner. STAT proteins are activated by ligand binding to cytokine receptors that associate with Janus kinase (JAK) family members.Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. STAT3 is activated downstream of numerous cytokines including interferons, IL-5, IL-6, IL-10, and LIF. STAT3 is important for the differentiation of Th17 cells and mediates a variety of cellular processes including cell growth and survival. The importance of STAT3 is highlighted by both loss-of-function and gain-of-function mutations. Deletion of STAT3 in T cells results in decreased IL-6- and IL-2-mediated proliferation, while deletion of STAT3 in neutrophils and macrophages results in increased susceptibility to LPS-induced endotoxic shock and increased production of the pro-inflammatory cytokines IL-6 and TNF alpha. Hyper STAT3 activity is associated with poor prognosis of many different cancers.Applications Reported: This LUVNKLA antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This LUVNKLA antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: Staining Intracellular Antigens for Flow Cytometry Protocol located in the Best Protocols Section under the Resources tab online.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.


Phospho-STAT3 (Y705)
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P40763, P42227
Signal Transducers and Activators of Transcription 3, Acute-phase response factor
Affinity chromatography
Flow Cytometry
IgG2b, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.

For Research Use Only.