Phospho-STAT1 (Tyr701), eFluor 450, clone: KIKSI0803, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-9008-41

Product Code. 15529826

Description

STAT1 (also known as signal transducers and activators of transcription) is a member of the STAT family of transcription factors. STAT proteins undergo phosphorylation in response to cytokines and growth factors and are translocated to the nucleus. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. The protein is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. The STAT1 gene is located on chromosome 2.

Description: This KIKSI0803 monoclonal antibody recognizes signal transducer and activator of transcription 1 (STAT1) when phosphorylated on tyrosine 701. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. Phosphorylation of STAT1 on tyrosine 701 by JAK1 and JAK2 is essential for STAT1 dimer formation, nuclear translocation, and DNA binding activity. In response to IFN gamma stimulation, STAT1 homodimerizes or forms heterodimers with STAT3 that can bind to GAS (IFN gamma-activated sequence) promoter elements. In response to either IFN alpha or IFN beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind ISRE (IFN-stimulated response element) promoter elements.Specificity of this KIKSI0803 clone was determined by ELISA, flow cytometry, and western blotting.Applications Reported: This KIKSI0803 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This KIKSI0803 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: Staining Intracellular Antigens for Flow Cytometry Protocol located in the Best Protocols Section under the Resources tab online.eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: Phospho-STAT1 (Tyr701)
• Classification: Monoclonal
• Concentration: 5μl/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P42224
• Gene Symbols: STAT1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6772
• Content And Storage: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Form: Liquid

• Applications: Flow Cytometry
• Clone: KIKSI0803
• Conjugate: eFluor 450
• Gene: STAT1
• Gene Alias: DKFZp686B04100, ISGF-3, STAT91
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.