Phospho-S6 (Ser235, Ser236), clone: cupk43k, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9007-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: This cupk43k monoclonal antibody recognizes human and mouse ribosomal protein S6 (also known as 40S ribosomal protein S6, phosphoprotein NP33, RPS6, RS6, S6) when phosphorylated on serine 235 (S235, human) and serine 236 (S236, mouse). Ribosomal protein S6 is a component of the 40S subunit of the ribosome and is phosphorylated at multiple sites following stimulation of cells by growth factors, tumor promoting agents, or mitogens. Phosphorylation of ribosomal protein S6 by p70S6K and PKDCD results in upregulation of the translation of RNA coding for proteins involved in cell cycle entry. Ribosomal protein S6 is dephosphorylated upon growth arrest.The specificity of the cupk43k monoclonal antibody was determined by western blotting.Applications Reported: This cupk43k antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated cupk43k is recommended for use in intracellular staining and flow cytometric analysis.).Applications Tested: This cupk43k antibody has been tested by immunoblotting of stimulated normal human peripheral blood cells. This can be used at less than or equal to 5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Phospho-S6 Ribosomal Protein (S235/S236)|
|PBS with 0.09% sodium azide; pH 7.2|
|40S ribosomal protein S6, Phosphoprotein NP33|
|Flow CytometryþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.