Invitrogen™ p38 MAPK (Phospho) [pT180/pY182] Multispecies InstantOne™ ELISA Kit

InstantOne ELISA

Brand:  Invitrogen™ 85-86022-11

Product Code. 15510777

Includes: User Manual
InstantOne™ Assay Plate - 96 well (sealed)
Cell Lysis Buffer (5X)
Enhancer Solution
Wash Buffer (10X)
phospho-p38 MAPK (Thr180/Tyr182) Capture Antibody Reagent
phospho-p38 MAPK (Thr180/Tyr182) Detection Antibody Reagent
Positive Control Cell Lysate (lyophilized)
Detection Reagent (TMB Substrate)
Stop Solution
Plate seal

Description

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human and mouse p38 MAPK in cell lysates. Detection of rat p38 MAPK is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

Specifications

• Accession Number: P47811,Q16539
• Product Type: ELISA Kit
• Target Species: Human, Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CSBP,CSBP1,CSBP2,CSPB1,EXIP,Mxi2,PRKM14,PRKM15,RK,SAPK2A,p38,p38ALPHA
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human, Mouse
• Total Assay Time: 1 hr. 10 min.

• Conjugate: Biotin
• Sample Type: Cell Lysates
• For Use With (Application): ELISA
• Gene ID (Entrez): 1432, 26416
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Quantity: 96 Tests
• Sample Volume: Cell Lysate,20 μL
• Synonym: SAPK2A, MAPK14
• Test Time: 1 hr. 20 min.

For Research Use Only