Phospho-NF kappa B p65 (S529) Mouse anti-Human, PE, Clone: B33B4WP, eBioscience™

Mouse Monoclonal Antibody

Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-9863-41

25TEST Anti-Human phospho-NF kappa B p65 (S529) PE

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg

Disclaimers: For Research Use Only.

Product Code. 15537256

Quantity Price
1 £110.90 / 25 tests
Estimated Shipment date
from Supplier 27-10-2016
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Description and Specification


Antigen Phospho-NF kappa B p65 (S529)
Applications Flow Cytometry (Intracellular Staining)
Clone B33B4WP
Concentration 5μL (0.06μg)/test
Conjugate PE
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Nuclear factor kappa B p65 subunit, RelA
Host Species Mouse
Isotype IgG2a
Quantity 25 tests
Regulatory Status RUO
Species Reactivity Human
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The B33B4WP monoclonal antibody recognizes human NF kappa B (NFkB) p65 subunit when serine 529 is phosphorylated. NFkB, also known as nuclear factor kappa-light chain enhancer of activated B cells, is a ubiquitous transcription factor that regulates the transcription of many genes involved in cell proliferation, apoptosis, development, immunity and cancer. Functional NFkB is a homo- or hetero-dimer composed of 5 members of the NFkB family: p65 (RelA), c-Rel, RelB, p50 (NFkB1, p105 precursor protein), and p52 (NFkB2, p100 precursor protein). The activity of the complex is negatively regulated by binding to IkB inhibitors that sequester NFkB into the cytoplasm, inhibiting its transcriptional activity. NFkB-activating agents like tumor necrosis factor (TNF) alpha, interleukin-1 beta, lipopolysaccharide, camptothecin, and phorbol ester (PMA) induce the phosphorylation and degradation of IkB, leading to the translocation of NFkB to the nucleus where it binds to kB motifs and regulates gene expression. The activity of p65-containing NFkB complexes is positively regulated by phosphorylation of the p65 subunit at serine 529 and/or serine 536.

The B33B4WP monoclonal antibody recognizes a single band at 65kDa in reduced SDS lysates from TNF alpha-stimulated HeLa cells.