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Phospho-NF kappa B p65 (S529) Mouse anti-Human, eFluor 660, Clone: B33B4WP, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9863-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15560157

  • £188.93 / 100 tests


Estimated Shipment date
from Supplier 30-08-2017
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Disclaimers:

For Research Use Only.


Specifications

Description

The B33B4WP monoclonal antibody recognizes human NF kappa B (NFkB) p65 subunit when serine 529 is phosphorylated. NFkB, also known as nuclear factor kappa-light chain enhancer of activated B cells, is a ubiquitous transcription factor that regulates the transcription of many genes involved in cell proliferation, apoptosis, development, immunity and cancer. Functional NFkB is a homo- or hetero-dimer composed of 5 members of the NFkB family: p65 (RelA), c-Rel, RelB, p50 (NFkB1, p105 precursor protein), and p52 (NFkB2, p100 precursor protein). The activity of the complex is negatively regulated by binding to IkB inhibitors that sequester NFkB into the cytoplasm, inhibiting its transcriptional activity. NFkB-activating agents like tumor necrosis factor (TNF) alpha, interleukin-1 beta, lipopolysaccharide, camptothecin, and phorbol ester (PMA) induce the phosphorylation and degradation of IkB, leading to the translocation of NFkB to the nucleus where it binds to kB motifs and regulates gene expression. The activity of p65-containing NFkB complexes is positively regulated by phosphorylation of the p65 subunit at serine 529 and/or serine 536.

The B33B4WP monoclonal antibody recognizes a single band at 65kDa in reduced SDS lysates from TNF alpha-stimulated HeLa cells.
Description & Specifications

Specifications

Phospho-NF kappa B p65 (S529)
B33B4WP
eFluor 660
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
5μL (0.06μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Nuclear factor kappa B p65 subunit, RelA
IgG2a
RUO
Primary
Human