Phospho-Mcl-1 (S159) Mouse anti-Human, Mouse, PE, Clone: RBCERNR, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-9038-42

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15577166

Quantity Price
1 £ 207.27 / 100 tests
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Isotype IgG2b
Conjugate PE
Antigen Phospho-Mcl-1 (S159)
Species Reactivity Human, Mouse
Primary or Secondary Primary
Quantity 100 tests
Clone RBCERNR
Format Conjugated
Host Species Mouse
Monoclonal or Polyclonal Monoclonal
Applications Flow Cytometry (Intracellular Staining)
Gene Alias BCL2L3, EAT
Concentration 5μL (0.125μg)/test
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Regulatory Status RUO

This RBCERNR monoclonal antibody recognizes human and mouse myeloid cell leukemia sequence 1 (Mcl-1) when phosphorylated on serine 159 (S159). Mcl-1 is an anti-apoptotic protein that is a member of the Bcl-2 family of proteins important for regulation of cell survival/apoptosis. Mcl-1 is primarily localized to the outer membrane of mitochondria where it prevents cytochrome c release via dimerization with other Bcl-2 family members such as Bim. PI3K activation of AKT results in the phosphorylation of GSK3 beta at serine 9 (S9) resulting in destabilization and degradation of GSK3 beta. Loss of GSK3 beta prevents phosphorylation of Mcl-1 on S159 and its subsequent ubiquitnation and degradation. Mice conditionally lacking Mcl-1 in lymphocytes showed that Mcl-1 is essential during early lymphoid development and for the maintenance of mature lymphocytes.