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Phospho-MCL-1 (Ser159), PE, clone: RBCERNR, eBioscience™

Mouse Monoclonal Antibody

£92.25 - £225.00

Specifications

Antigen Phospho-MCL-1 (Ser159)
Clone RBCERNR
Target Species Human, Murine
Conjugate PE
Isotype IgG2b, kappa, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15567166
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Affymetrix eBioscience
12-9038-41
25 Tests
£92.25
25 tests
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Estimated Shipment
13-01-2021
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15577166
View Documents
Affymetrix eBioscience
12-9038-42
100 Tests
£225.00
100 tests
View alternative products
Save Up to 
»
Estimated Shipment
13-01-2021
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Description: This RBCERNR monoclonal antibody recognizes human and mouse myeloid cell leukemia sequence 1 (Mcl-1) when phosphorylated on serine 159 (S159). Mcl-1 is an anti-apoptotic protein that is a member of the Bcl-2 family of proteins important for regulation of cell survival/apoptosis. Mcl-1 is primarily localized to the outer membrane of mitochondria where it prevents cytochrome c release via dimerization with other Bcl-2 family members such as Bim. PI3K activation of AKT results in the phosphorylation of GSK3 beta at serine 9 (S9) resulting in destabilization and degradation of GSK3 beta. Loss of GSK3 beta prevents phosphorylation of Mcl-1 on S159 and its subsequent ubiquitnation and degradation. Mice conditionally lacking Mcl-1 in lymphocytes showed that Mcl-1 is essential during early lymphoid development and for the maintenance of mature lymphocytes.Applications Reported: This RBCERNR antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This RBCERNR antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Staining Protocol: Protocol A and Protocol C are recommended for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to Clone Performance Following Fixation/Permeabilization located in the Best Protocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: Staining Intracellular Antigens for Flow Cytometry Protocol located in the Best Protocols Section under the Resources tab online.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Phospho-MCL-1 (Ser159)
Human, Murine
IgG2b, kappa, kappa
Monoclonal
Liquid
Antibody
Store at 2-8°C. Do not freeze. Light-sensitive material.
RBCERNR
PE
Flow Cytometry
Primary
Monoclonal
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.