Phospho-LCK (Y505) Mouse anti-Human, Mouse, PerCP-eFluor 710, Clone: SRRCHA, eBioscience™

Mouse Monoclonal Antibody

Brand: Affymetrix eBioscience

Manufacturer Part Number: 46-9076-41

25TEST Anti-Human/Mouse phospho-LCK (Y505) PerCP-eFluor 710

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg

Disclaimers: For Research Use Only.

Product Code. 15549396

Quantity Price
1 £129.50 / 25 tests
Estimated Shipment date
from Supplier 01-11-2016
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Description and Specification


Antigen Phospho-LCK (Y505)
Applications Flow Cytometry (Intracellular Staining)
Concentration 5μL (0.06μg)/test
Conjugate PerCP-eFluor 710
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias lymphocyte-specific protein tyrosine kinase, Leukocyte C-terminal Src kinase, p56lck
Host Species Mouse
Isotype IgG1
Quantity 25 tests
Regulatory Status RUO
Species Reactivity Human, Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

This SRRCHA monoclonal antibody recognizes human and mouse LCK when phosphorylated on tyrosine 505 (Y505). Y505 is a negative-regulatory site that is phosphorylated by the kinase CSK. LCK is expressed in T cells where it associates with the cytoplasmic tails of the CD4 and CD8 co-receptors on T helper cells and cytotoxic T cells, respectively. When the TCR is engaged, LCK acts to phosphorylate the intracellular chains of CD3 and zeta chains of the TCR complex, allowing the cytoplasmic tyrosine kinase, ZAP-70, to bind to them. LCK then phosphorylates and activates ZAP-70, which then phosphorylates the adaptor protein, LAT, that serves as a docking site for a number of other proteins, including SLP-76, Grb2, and phospholipase C gamma1. Mice lacking expression of LCK show defects in T cell development and function.