Phospho-LCK (Y505) Mouse anti-Human, Mouse, PerCP-eFluor 710, Clone: SRRCHA, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 46-9076-41

25TEST Anti-Human/Mouse phospho-LCK (Y505) PerCP-eFluor 710

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15549396

Quantity Price
1 £ 129.5 / 25 tests
Estimated Shipment date
from Supplier 21-02-2017
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Description and Specification

Specification

Primary or Secondary Primary
Antigen Phospho-LCK (Y505)
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias lymphocyte-specific protein tyrosine kinase, Leukocyte C-terminal Src kinase, p56lck
Host Species Mouse
Applications Flow Cytometry (Intracellular Staining)
Isotype IgG1
Quantity 25 tests
Regulatory Status RUO
Species Reactivity Human, Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Monoclonal or Polyclonal Monoclonal
Clone SRRCHA
Concentration 5μL (0.06μg)/test
Conjugate PerCP-eFluor 710

This SRRCHA monoclonal antibody recognizes human and mouse LCK when phosphorylated on tyrosine 505 (Y505). Y505 is a negative-regulatory site that is phosphorylated by the kinase CSK. LCK is expressed in T cells where it associates with the cytoplasmic tails of the CD4 and CD8 co-receptors on T helper cells and cytotoxic T cells, respectively. When the TCR is engaged, LCK acts to phosphorylate the intracellular chains of CD3 and zeta chains of the TCR complex, allowing the cytoplasmic tyrosine kinase, ZAP-70, to bind to them. LCK then phosphorylates and activates ZAP-70, which then phosphorylates the adaptor protein, LAT, that serves as a docking site for a number of other proteins, including SLP-76, Grb2, and phospholipase C gamma1. Mice lacking expression of LCK show defects in T cell development and function.