Phospho-LCK (Y505) Mouse anti-Human, Mouse, PerCP-eFluor 710, Clone: SRRCHA, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 46-9076-41

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15549396

Quantity Price
1 £ 84.99 / 25 tests
Estimated Shipment date
from Supplier 08-05-2017
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Description and Specification

Specification

Primary or Secondary Primary
Quantity 25 tests
Clone SRRCHA
Regulatory Status RUO
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Format Conjugated
Concentration 5μL (0.06μg)/test
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Mouse
Antigen Phospho-LCK (Y505)
Isotype IgG1
Gene Alias lymphocyte-specific protein tyrosine kinase, Leukocyte C-terminal Src kinase, p56lck
Conjugate PerCP-eFluor 710
Monoclonal or Polyclonal Monoclonal
Applications Flow Cytometry (Intracellular Staining)
Species Reactivity Human, Mouse

This SRRCHA monoclonal antibody recognizes human and mouse LCK when phosphorylated on tyrosine 505 (Y505). Y505 is a negative-regulatory site that is phosphorylated by the kinase CSK. LCK is expressed in T cells where it associates with the cytoplasmic tails of the CD4 and CD8 co-receptors on T helper cells and cytotoxic T cells, respectively. When the TCR is engaged, LCK acts to phosphorylate the intracellular chains of CD3 and zeta chains of the TCR complex, allowing the cytoplasmic tyrosine kinase, ZAP-70, to bind to them. LCK then phosphorylates and activates ZAP-70, which then phosphorylates the adaptor protein, LAT, that serves as a docking site for a number of other proteins, including SLP-76, Grb2, and phospholipase C gamma1. Mice lacking expression of LCK show defects in T cell development and function.