Phospho-Histone H2A.X (Ser139), PerCP-eFluor™ 710, clone: CR55T33, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 46-9865-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The CR55T33 monoclonal antibody recognizes phosphorylated serine 139 of human and mouse H2AX. H2AX is a member of the H2A histone family that complex with DNA and other histones to form the repeating nucleosome units characteristic of eukaryotic chromatin. Nucleosomes consist of approximately 147 base pairs of DNA wrapped around an octamer of histones composed of two each of the four histone proteins: H2A, H2B, H3 and H4. After induction of DNA damage such as double-strand breaks by irradiation, genotoxic stresses, replication errors or gene recombination, PI3K-like kinases (e.g., ataxia telangiectasia mutated (ATM), ataxia telangiectasia Rad-3-related (ATR), and DNA-dependent protein kinase (DNA-PK) are activated to phosphorylate serine 139 in H2AX. This early phosphorylation event plays a critical role in recruiting proteins involved in DNA repair.The monoclonal antibody CR55T33 recognizes a single band of approximately 15kDa on reduced cell lysates from Jurkat cells stimulated with etoposide.Applications Reported: This CR55T33 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This CR55T33 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of treated human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol protocol. Refer to Best Protocols; Staining intracellular Antigens for Flow Cytometry Protocol (Protocol B: One step protocol for (nuclear) intracellular proteins). This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Histone H2A type 2-C, H2A 2C, H2AFQ, HIST2H2AC, Histone H2A/q|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.