Phospho-H2A.x (S139) Mouse anti-Human, Mouse, eFluor 660, Clone: CR55T33, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 50-9865-42

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15580157

Quantity Price
1 £ 188.93 / 100 tests
Estimated Shipment date
from Supplier 09-05-2017
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Description and Specification

Specification

Primary or Secondary Primary
Quantity 100 tests
Applications Flow Cytometry (Intracellular Staining)
Applications Immunocytochemistry
Isotype IgG1
Monoclonal or Polyclonal Monoclonal
Species Reactivity Human, Mouse
Clone CR55T33
Gene Alias Histone H2AX
Format Conjugated
Host Species Mouse
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Regulatory Status RUO
Conjugate eFluor 660
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Concentration 0.2mg/mL; 5μL (0.125μg)/test
Antigen Phospho-H2A.x (S139)

The CR55T33 monoclonal antibody recognizes phosphorylated serine 139 of human and mouse H2AX. H2AX is a member of the H2A histone family that complex with DNA and other histones to form the repeating nucleosome units characteristic of eukaryotic chromatin. Nucleosomes consist of approximately 147 base pairs of DNA wrapped around an octamer of histones composed of two each of the four histone proteins: H2A, H2B, H3 and H4. After induction of DNA damage such as double-strand breaks by irradiation, genotoxic stresses, replication errors or gene recombination, PI3K-like kinases (e.g., ataxia telangiectasia mutated (ATM), ataxia telangiectasia Rad-3-related (ATR), and DNA-dependent protein kinase (DNA-PK)) are activated to phosphorylate serine 139 in H2AX. This early phosphorylation event plays a critical role in recruiting proteins involved in DNA repair.

The monoclonal antibody CR55T33 recognizes a single band of approximately 15kDa on reduced cell lysates from Jurkat cells stimulated with etoposide.