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Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (CR55T33), eFluor™ 660, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 50-9865-42
168.00 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.
Code : NEW
Additional Details : Weight : 0.23750kg
Description
Description: The CR55T33 monoclonal antibody recognizes phosphorylated serine 139 of human and mouse H2AX. H2AX is a member of the H2A histone family that complex with DNA and other histones to form the repeating nucleosome units characteristic of eukaryotic chromatin. Nucleosomes consist of approximately 147 base pairs of DNA wrapped around an octamer of histones composed of two each of the four histone proteins: H2A, H2B, H3 and H4. After induction of DNA damage such as double-strand breaks by irradiation, genotoxic stresses, replication errors or gene recombination, PI3K-like kinases (e.g., ataxia telangiectasia mutated (ATM), ataxia telangiectasia Rad-3-related (ATR), and DNA-dependent protein kinase (DNA-PK)) are activated to phosphorylate serine 139 in H2AX. This early phosphorylation event plays a critical role in recruiting proteins involved in DNA repair. The monoclonal antibody CR55T33 recognizes a single band of approximately 15 kDa on reduced cell lysates from Jurkat cells stimulated with etoposide. Applications Reported: This CR55T33 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunocytochemistry. Applications Tested: This CR55T33 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of treated human peripheral blood cells using the Foxp3/Transcirption Factor Buffer Set (cat. 00-5523) and protocol. This can be used at 5 μL (0.
Phospho-Histone H2A.X (Ser139) Monoclonal antibody specifically detects Phospho-Histone H2A.X (Ser139) in Human, Mouse samples. It is validated for Flow Cytometry, Immunocytochemistry.Specifications
Phospho-Histone H2A.X (Ser139) | |
Monoclonal | |
5 μL/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
P16104, P27661 | |
H2AX | |
Affinity chromatography | |
RUO | |
15270, 3014 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry, Immunocytochemistry | |
CR55T33 | |
eFluor 660 | |
H2AX | |
AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2A.X variant histone; H2A/X; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329 | |
Mouse | |
100 Tests | |
Primary | |
Human, Mouse | |
Antibody | |
IgG1 κ |
For Research Use Only.