Phospho-H2A.x (S139) Mouse anti-Human, Mouse, Clone: CR55T33, eBioscience™

Mouse Monoclonal Antibody

Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-9865-80

25UG Anti-Human/Mouse phospho-H2AX (S139) Purified

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg

Disclaimers: For Research Use Only.

Product Code. 15567696

Quantity Price
1 £101.60 / 25µg
Estimated Shipment date
from Supplier 27-10-2016
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Description and Specification


Antigen Phospho-H2A.x (S139)
Applications Flow Cytometry (Intracellular Staining)
Applications Immunocytochemistry
Applications Immunohistochemistry (Formalin/Paraffin)
Applications Western Blotting
Clone CR55T33
Concentration 0.5mg/mL
Conjugate Unlabeled
Format Purified
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Histone H2AX
Host Species Mouse
Isotype IgG1
Quantity 25μg
Regulatory Status RUO
Species Reactivity Human, Mouse
Storage Requirements Store at 2-8°C.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The CR55T33 monoclonal antibody recognizes phosphorylated serine 139 of human and mouse H2AX. H2AX is a member of the H2A histone family that complex with DNA and other histones to form the repeating nucleosome units characteristic of eukaryotic chromatin. Nucleosomes consist of approximately 147 base pairs of DNA wrapped around an octamer of histones composed of two each of the four histone proteins: H2A, H2B, H3 and H4. After induction of DNA damage such as double-strand breaks by irradiation, genotoxic stresses, replication errors or gene recombination, PI3K-like kinases (e.g., ataxia telangiectasia mutated (ATM), ataxia telangiectasia Rad-3-related (ATR), and DNA-dependent protein kinase (DNA-PK) are activated to phosphorylate serine 139 in H2AX. This early phosphorylation event plays a critical role in recruiting proteins involved in DNA repair.

The monoclonal antibody CR55T33 recognizes a single band of approximately 15kDa on reduced cell lysates from Jurkat cells stimulated with etoposide.