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Phospho-ERK1/2 (Thr202, Tyr204), Alexa Fluor 488, clone: MILAN8R, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-9109-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15530267

  • £81.11 / 25 tests


Estimated Shipment date
from Supplier 23-01-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This MILAN8R monoclonal antibody recognizes human and mouse extracellular signal-regulated kinases 1 and 2 (also known as ERK1/2, p44/p42, or MAPK3/1) when phosphorylated on T202/Y204. ERK1/2 belong to a family of conserved serine/threonine protein kinases known as mitogen-activated protein kinases (MAPKs) that are involved in many cellular programs such as proliferation, differentiation, motility, and survival. ERK1/2 signaling is activated in response to numerous extracellular stimuli including mitogens, growth factors, and cytokines. The primary activators of ERK1/2 are MEK1 and MEK2 which act by phosphorylating the activation loop residues T202/Y204 and T185/Y187 in ERK1 and ERK2, respectively. Several downstream targets of ERK1/2 have been identified, including p90RSK and the transcription factor Elk-1. ERK1/2 are negatively regulated by MAPK phosphatases, known as DUSPs or MKPs, as well as by chemical inhibitors of MEK including U0126 and PD98059. Disruption of the ERK pathway is common in many types of cancer. Specificity of this MILAN8R clone was determined by ELISA, flow cytometry, and western blotting.Applications Reported: This MILAN8R antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MILAN8R antibody has been tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: Staining Intracellular Antigens for Flow Cytometry Protocol located in the Best Protocols Section under the Resources tab online.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Phospho-ERK1/2 (T202/Y204)
MILAN8R
Alexa Fluor 488
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
MAPK, ERK, p42/p44
IgG1
RUO
Primary
Murine
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