Phospho-ERK1/2 (Thr202, Tyr204), clone: MILAN8R, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9109-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: This MILAN8R monoclonal antibody recognizes human and mouse extracellular signal-regulated kinases 1 and 2 (also known as ERK1/2, p44/p42, or MAPK3/1) when phosphorylated on T202/Y204. ERK1/2 belong to a family of conserved serine/threonine protein kinases known as mitogen-activated protein kinases (MAPKs) that are involved in many cellular programs such as proliferation, differentiation, motility, and survival. ERK1/2 signaling is activated in response to numerous extracellular stimuli including mitogens, growth factors, and cytokines. The primary activators of ERK1/2 are MEK1 and MEK2 which act by phosphorylating the activation loop residues T202/Y204 and T185/Y187 in ERK1 and ERK2, respectively. Several downstream targets of ERK1/2 have been identified, including p90RSK and the transcription factor Elk-1. ERK1/2 are negatively regulated by MAPK phosphatases, known as DUSPs or MKPs, as well as by chemical inhibitors of MEK including U0126 and PD98059. Disruption of the ERK pathway is common in many types of cancer.Applications Reported: This MILAN8R antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry. (Fluorochrome conjugated MILAN8R is recommended for use in intracellular flow cytometry.).Applications Tested: This MILAN8R antibody has been tested by immunoblotting of stimulated Jurkat cells and can be used at less than or equal to 5 μg/mL. This MILAN8R antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and by immunocytochemistry of MeOH-fixed cells and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Phospho-ERK 1/2 (T202/Y204)|
|PBS with 0.09% sodium azide; pH 7.2|
|P27361, P28482, P63085, Q63844|
|Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2 , MAPK1, ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3, p44, p42|
|Flow CytometryþImmunocytochemistryþImmunohistochemistryþImmunohistochemistryþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.