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Phospho-CHK2 (Thr68), PE, clone: ebchk2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9508-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15537216

  • £208.13 / 100 tests


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Description

Description

Description: This ebchk2 monoclonal antibody recognizes human and mouse Checkpoint Kinase 2 (Chk2) when phosphorylated on threonine 68 (T68). Chk2 is a cell cycle checkpoint regulator and tumor suppressor. Along with Chk1, it plays a critical role in the cellular response to DNA damage, and is particularly important during G2/M transition. Chk2 is activated via phosphorylation on T68 by the kinase ATM, which responds primarily to double-stranded DNA breaks. Upon activation, Chk2 functions include inhibition of Cdc25 family phosphatases and activation of p53, both of which prevent progression of the cell cycle. Abnormal regulation of the ATM-Chk2 pathway is often observed in cancer.Applications Reported: This ebchk2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This ebchk2 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to Clone Performance Following Fixation/Permeabilization located in the Best Protocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: Staining Intracellular Antigens for Flow Cytometry Protocol located in the Best Protocols Section under the Resources tab online.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

phospho Chk2
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q9Z265, O96017
CDS1, CHEK2, HuCds1, PP1425, RAD53
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
HumanĂ¾Murine
Flow Cytometry
ebchk2
PE
CHEK2
Conjugated
Chek2
IgG2b, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
11200
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