Phospho-BTK/ITK (Y551/Y511) Mouse anti-Human, Mouse, PerCP-eFluor 710, Clone: M4G3LN, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 46-9015-41

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15509396

Quantity Price
1 £ 91.11 / 25 tests
Estimated Shipment date
from Supplier 03-05-2017
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Description and Specification

Specification

Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Format Conjugated
Concentration 5μL (0.5μg)/test
Host Species Mouse
Conjugate PerCP-eFluor 710
Antigen Phospho-BTK/ITK (Y551/Y511)
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Regulatory Status RUO
Isotype IgG2b
Primary or Secondary Primary
Quantity 25 tests
Gene Alias Bruton’s tyrosine kinase, IL-2 inducible T-cell kinase, p-BTK/ITK, phospho-BTK/ITK, pBTK/ITK
Species Reactivity Human, Mouse
Clone M4G3LN
Applications Flow Cytometry (Intracellular Staining)
Monoclonal or Polyclonal Monoclonal

This M4G3LN monoclonal antibody recognizes human and mouse Bruton’s tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively.

In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.

Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting.