Phospho-BTK/ITK (Y551/Y511) Mouse anti-Human, Mouse, eFluor 660, Clone: M4G3LN, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 50-9015-82

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15530067

Quantity Price
1 £ 213.39 / 100µg
Estimated Shipment date
from Supplier 03-04-2017
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Description and Specification

Specification

Host Species Mouse
Applications Immunohistochemistry
Applications Immunohistochemistry (Formalin/Paraffin)
Species Reactivity Human, Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Clone M4G3LN
Antigen Phospho-BTK/ITK (Y551/Y511)
Isotype IgG2b
Regulatory Status RUO
Monoclonal or Polyclonal Monoclonal
Conjugate eFluor 660
Quantity 100μg
Concentration 0.2mg/mL
Format Conjugated

This M4G3LN monoclonal antibody recognizes human and mouse Bruton’s tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively.

In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.

Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting.