missing translation for 'onlineSavingsMsg'
Learn More

Phospho-BTK/ITK (Tyr551, Tyr511), clone: M4G3LN, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9015-80

Code : NEW

Additional Details : Weight : 0.09500kg

 View more versions of this product

Product Code. 15547566

  • £93.58 / 25µg


Estimated Shipment date
from Supplier 26-02-2018
Add to basket

Disclaimers:

For Research Use Only.


Description

Description

Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton's tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively.In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting.Applications Reported: This M4G3LN antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated M4G3LN is recommended for use in intracellular flow cytometry.).Applications Tested: This M4G3LN antibody has been tested by immunoblotting of stimulated Daudi B cells. This can be used at less than or equal to 5 μg/mL. This antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 0.1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Phospho-BTK/ITK (Y551/Y511)
M4G3LN
Unconjugated
Purified
Murine
25μg
Store at 2-8°C.
Monoclonal
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Bruton’s tyrosine kinase (Btk), agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase/IL-2 inducible T-cell kinase (Itk), T-cell-specific kinase, Tyrosine-protein kinase Lyk
IgG2b
RUO
Primary
Murine
Videos