Phospho-BTK/ITK (Y551/Y511) Mouse anti-Human, Mouse, Clone: M4G3LN, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-9015-80

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15547566

Quantity Price
1 £ 91.11 / 25µg
Estimated Shipment date
from Supplier 09-05-2017
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Description and Specification

Specification

Isotype IgG2b
Conjugate Unlabeled
Antigen Phospho-BTK/ITK (Y551/Y511)
Storage Requirements Store at 2-8°C.
Applications Flow Cytometry (Intracellular Staining)
Applications Immunohistochemistry (Formalin/Paraffin)
Applications Western Blotting
Host Species Mouse
Regulatory Status RUO
Clone M4G3LN
Quantity 25μg
Format Purified
Monoclonal or Polyclonal Monoclonal
Species Reactivity Human, Mouse
Primary or Secondary Primary
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Bruton’s tyrosine kinase (Btk), agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase/IL-2 inducible T-cell kinase (Itk), T-cell-specific kinase, Tyrosine-protein kinase Lyk
Concentration 0.5mg/mL

This M4G3LN monoclonal antibody recognizes human and mouse Bruton’s tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively.

In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.

Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting.