Phospho-BTK/ITK (Tyr551, Tyr511), clone: M4G3LN, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9015-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: This M4G3LN monoclonal antibody recognizes human and mouse Bruton's tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively.In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting.Applications Reported: This M4G3LN antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated M4G3LN is recommended for use in intracellular flow cytometry.).Applications Tested: This M4G3LN antibody has been tested by immunoblotting of stimulated Daudi B cells. This can be used at less than or equal to 5 μg/mL. This antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 0.1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Q06187, Q08881, Q03526, P35991|
|agammaglobulinaemia tyrosine kinase, AGMX1, ATK, B-cell progenitor kinase, BPK, Bruton agammaglobulinemia tyrosine kinase, Bruton's tyrosine kinase, dominant-negative kinase-deficient Brutons tyrosine kinase, truncated Bruton agammaglobulinemia tyrosine kinase, tyrosine-protein kinase BTK isoform (lacking exon 13 to 17), tyrosine-protein kinase BTK isoform (lacking exon 14), EMT, LYK, PSCTK2, IL-2-inducible T cell kinase, homolog of mouse T-cell itk/tsk, IL-2-inducible T cell kinase, IL-2-inducible T-cell kinase, IL2-inducible T-cell kinase, interleukin-2-inducible T cell kinase, interleukin-2-inducible T-cell kinase, kinase EMT, T-cell-specific kinase, tyrosine-protein kinase ITK/TSK, tyrosine-protein kinase LYK|
|Flow CytometryþImmunohistochemistryþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.