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Phospho-BTK/ITK (Tyr551, Tyr511), APC, clone: M4G3LN, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-9015-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15588216

  • £86.94 / 25 tests


EU Stock 2
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Description

Description

Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton's tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively.In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting.Applications Reported: This M4G3LN antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This M4G3LN antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to Clone Performance Following Fixation/Permeabilization located in the Best Protocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: Staining Intracellular Antigens for Flow Cytometry Protocol located in the Best Protocols Section under the Resources tab online.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Phospho-BTK/ITK (Y551/Y511)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q06187, Q08881, Q03526, P35991
agammaglobulinaemia tyrosine kinase, AGMX1, ATK, B-cell progenitor kinase, BPK, Bruton agammaglobulinemia tyrosine kinase, Bruton's tyrosine kinase, dominant-negative kinase-deficient Brutons tyrosine kinase, truncated Bruton agammaglobulinemia tyrosine kinase, tyrosine-protein kinase BTK isoform (lacking exon 13 to 17), tyrosine-protein kinase BTK isoform (lacking exon 14), EMT, LYK, PSCTK2, IL-2-inducible T cell kinase, homolog of mouse T-cell itk/tsk, IL-2-inducible T cell kinase, IL-2-inducible T-cell kinase, IL2-inducible T-cell kinase, interleukin-2-inducible T cell kinase, interleukin-2-inducible T-cell kinase, kinase EMT, T-cell-specific kinase, tyrosine-protein kinase ITK/TSK, tyrosine-protein kinase LYK
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
HumanĂ¾Murine
Flow Cytometry
M4G3LN
APC
ITK
Conjugated
BTK, ITK
IgG2b, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3702, 695
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