Phospho-ATM (Ser1981), clone: 10H11.E12, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9046-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: This 10H11.E12 monoclonal antibody recognizes the human, mouse, and rat ataxia-telangiectasia (ATM) protein when phosphorylated on serine 1981. ATM belongs to the family of PI3 kinases and functions by coordinating cell cycle arrest and initiation of DNA repair through the phosphorylation of a multitude of substrates in response to DNA damage and oxidative stress. ATM exists as dimers or multimers in its inactive state but in response to DNA breaks, becomes activated through formation of monomers and autophosphorylation. Activated ATM is recruited to the site of DNA double strand breaks where it halts the cell cycle and initiates DNA repair through the phosphorylation of downstream DNA damage response pathway members. Loss of functional activity of ATM results in ataxia-telangiectasia (AT), a disease characterized by early onset neurodegeneration and predisposition to cancer. AT patients are immunodeficient, radiosensitive, and have an increased risk of certain cancer types such as lymphoma and leukemia.Applications Reported: This 10H11.E12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, western blotting, immunocytochemistry, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated 10H11.E12 is recommended for use in intracellular flow cytometry.).Applications Tested: This 10H11.E12 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using either high or low pH antigen retrieval and can be used at less than or equal to 10 μg/mL. This 10H11.E12 antibody has been tested by immunocytochemistry of methanol or formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|