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Phospho-ATM (Ser1981) Mouse anti-Human, Mouse, Rat, Clone: 10H11.E12, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9046-80

Code : NEW

Additional Details : Weight : 0.09500kg

Product Code. 15577566

  • £67.75 / 25µg

Estimated Shipment
26-10-2020

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Description

Description

E12 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using either high or low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. This 10H11.E12 antibody has been tested by immunocytochemistry of methanol or formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM’s C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.
Specifications

Specifications

Phospho-ATM (Ser1981)
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q13315, Q62388
AT1, ATA, ATC, ATD, ATDC, ATE, DKFZp781A0353, MGC74674
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse, Rat
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
10H11.E12
Unconjugated
ATM
Liquid
ATM
IgG1, kappa
25 μg
4° C
Primary
11920, 300711, 472
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