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Phospho-ATM (Ser1981), clone: 10H11.E12, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9046-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15577566

  • £63.84 / 25µg


Estimated Shipment date
from Supplier 26-06-2018
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Description

Description

Description: This 10H11.E12 monoclonal antibody recognizes the human, mouse, and rat ataxia-telangiectasia (ATM) protein when phosphorylated on serine 1981. ATM belongs to the family of PI3 kinases and functions by coordinating cell cycle arrest and initiation of DNA repair through the phosphorylation of a multitude of substrates in response to DNA damage and oxidative stress. ATM exists as dimers or multimers in its inactive state but in response to DNA breaks, becomes activated through formation of monomers and autophosphorylation. Activated ATM is recruited to the site of DNA double strand breaks where it halts the cell cycle and initiates DNA repair through the phosphorylation of downstream DNA damage response pathway members. Loss of functional activity of ATM results in ataxia-telangiectasia (AT), a disease characterized by early onset neurodegeneration and predisposition to cancer. AT patients are immunodeficient, radiosensitive, and have an increased risk of certain cancer types such as lymphoma and leukemia.Applications Reported: This 10H11.E12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, western blotting, immunocytochemistry, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated 10H11.E12 is recommended for use in intracellular flow cytometry.).Applications Tested: This 10H11.E12 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using either high or low pH antigen retrieval and can be used at less than or equal to 10 μg/mL. This 10H11.E12 antibody has been tested by immunocytochemistry of methanol or formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Phospho-ATM (S1981)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q13315, Q62388
AT1, ATA, ATC, ATD, ATDC, ATE, DKFZp781A0353, MGC74674
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Rat
Western Blot
10H11.E12
Unconjugated
ATM
Purified
ATM
IgG1, kappa
25μg
Store at 2-8°C.
Primary
472
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