Phospho-AKT (S473) Mouse anti-Human, Mouse, APC, Clone: SDRNR, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 17-9715-42

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15558256

Quantity Price
1 £ 211.35 / 100 tests
EU Stock 9
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Description and Specification

Specification

Conjugate APC
Host Species Mouse
Isotype IgG2a
Applications Flow Cytometry (Intracellular Staining)
Format Conjugated
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Antigen Phospho-AKT (S473)
Concentration 5μL (0.5μg)/test
Gene Alias Protein kinase B (PKB)
Species Reactivity Human, Mouse
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Quantity 100 tests
Regulatory Status RUO
Primary or Secondary Primary
Clone SDRNR
Monoclonal or Polyclonal Monoclonal

This SDRNR monoclonal antibody recognizes human and mouse AKT (also known as Protein Kinase B (PKB)) when phosphorylated on S473. AKT is a serine/threonine protein kinase that plays a key role in multiple cellular processes including metabolism, proliferation, apoptosis/survival, and migration. There are three homologous isoforms of AKT: AKT1, AKT2, and AKT3. AKT is activated by binding of its pleckstrin homology (PH) domain to membrane phospholipids and by phosphorylation. Phosphorylation of AKT at T308 by PDK1 and at S473 is required for full activation of this kinase. AKT promotes cell survival by inhibiting apoptosis via phosphorylation and inactivation of several targets including Bad, Foxo1, c-Raf, and caspase-9. Deregulation of AKT has been implicated as a major contributing factor in many types of cancer. AKT is negatively regulated by the phosphatase PTEN as well as by the chemical inhibitor LY294002. Specificity of this SDRNR clone was determined by ELISA, flow cytometry, and western blotting.