Phospho-AKT (S473) Mouse anti-Human, Mouse, APC, Clone: SDRNR, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 17-9715-41

25TEST Anti-Human/Mouse phospho-AKT (S473) APC

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15548256

Quantity Price
1 £ 129.5 / 25 tests
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

Antigen Phospho-AKT (S473)
Applications Flow Cytometry (Intracellular Staining)
Clone SDRNR
Concentration 5μL (0.5μg)/test
Conjugate APC
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Protein kinase B (PKB)
Host Species Mouse
Isotype IgG2a
Quantity 25 tests
Regulatory Status RUO
Species Reactivity Human, Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

This SDRNR monoclonal antibody recognizes human and mouse AKT (also known as Protein Kinase B (PKB)) when phosphorylated on S473. AKT is a serine/threonine protein kinase that plays a key role in multiple cellular processes including metabolism, proliferation, apoptosis/survival, and migration. There are three homologous isoforms of AKT: AKT1, AKT2, and AKT3. AKT is activated by binding of its pleckstrin homology (PH) domain to membrane phospholipids and by phosphorylation. Phosphorylation of AKT at T308 by PDK1 and at S473 is required for full activation of this kinase. AKT promotes cell survival by inhibiting apoptosis via phosphorylation and inactivation of several targets including Bad, Foxo1, c-Raf, and caspase-9. Deregulation of AKT has been implicated as a major contributing factor in many types of cancer. AKT is negatively regulated by the phosphatase PTEN as well as by the chemical inhibitor LY294002. Specificity of this SDRNR clone was determined by ELISA, flow cytometry, and western blotting.