phospho-AKT Mouse, FITC, Clone: SDRNR, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 11-9715-41

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15576626

Quantity Price
1 £ 72.77 / 25 tests
EU Stock 1
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Description and Specification

Specification

Concentration 5μL (0.06μg)/test
Clone SDRNR
Isotype IgG2a
Regulatory Status RUO
Primary or Secondary Primary
Conjugate FITC
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Species Reactivity Human , Mouse
Applications Flow Cytometry (Intracellular Staining)
Gene Alias Protein Kinase B (PKB)
Monoclonal or Polyclonal Monoclonal
Antigen phospho-AKT
Quantity 25 tests
Format Conjugated

This SDRNR monoclonal antibody recognizes human and mouse AKT (also known as Protein Kinase B (PKB)) when phosphorylated on S473. AKT is a serine/threonine protein kinase that plays a key role in multiple cellular processes including metabolism, proliferation, apoptosis/survival, and migration. There are three homologous isoforms of AKT: AKT1, AKT2, and AKT3. AKT is activated by binding of its pleckstrin homology (PH) domain to membrane phospholipids and by phosphorylation. Phosphorylation of AKT at T308 by PDK1 and at S473 is required for full activation of this kinase. AKT promotes cell survival by inhibiting apoptosis via phosphorylation and inactivation of several targets including Bad, Foxo1, c-Raf, and caspase-9. Deregulation of AKT has been implicated as a major contributing factor in many types of cancer. AKT is negatively regulated by the phosphatase PTEN as well as by the chemical inhibitor LY294002. Specificity of this SDRNR clone was determined by ELISA, flow cytometry, and western blotting.