Invitrogen™ PGFM Enzyme Competitive ELISA Kit

Competitive ELISA

Brand:  Invitrogen™ EIAPGFM

Product Code. 15815863

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.

Description

The PGFM Enzyme ELISA quantitates PGFM in serum,plasma,urine,dried fecal extracts,or cell culture medium.
Principle of the method
The PGFM Competitive ELISA research-use-only kit designed to quantitatively measure PGFM present in fecal extracts,urine,serum,and plasma samples. A PGFM standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit IgG. A PGFM-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody highly specific to PGFM to each well. After a 1-hour incubation,the plate is washed and substrate is added. The substrate reacts with the bound PGFM-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

In many species, uterine and placental Prostaglandin F2alpha (PGFM) is involved in the regulation of reproductive and pregnancy-related processes such as embryonic development, initiation of parturition, and resumption of ovarian activity. Prostaglandin F2alpha is metabolized to PGFM during the first passage through the lungs. PGFM has a longer half-life in peripheral circulation than PGF2alpha and has been applied as a useful analytical marker of PGF2alpha. PGFM is identical across all species and so this kit should measure PGFM from sources other than those tested.

Specifications

• Assay Range: 50-3200 pg/mL
• Description: PGFM Competitive ELISA Kit
• Conjugate: HRP
• Product Type: ELISA Kit
• Sample Type: Fecal Extract, Plasma, Serum, Supernatant, Urine
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Intraassay CV: 9.2%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Fecal Extract,50 μL; Plasma, 6.25 μL; Serum, 6.25 μL; Supernatant, 50 μL; Urine,6.25 μL
• Target: Chemical
• Total Assay Time: 1 hr. 30 min.

• Assay Sensitivity: 20.8 pg/mL
• Assay: Multiplex assay on the Luminex Platform
• Detection Method: Fluorescence
• Protein Family: Prostaglandin
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• Interassay CV: 9.7%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Reproductive Biology
• Storage Requirements: -20°C
• Test Time: 1 hr. 20 min.