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Perforin, PerCP-eFluor™ 710, clone: dG9 (delta G9), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9994-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15549486

  • £231.47 / 100 tests


EU Stock 2
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Description

Description

Description: The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.Applications Reported: This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This dG9 (delta G9) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Perforin
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P14222
cytolysin, FLH2, HPLH2, lymphocyte pore forming protein, lymphocyte pore-forming protein, perforin 1 (pore forming protein), perforin-1, PFN1, PFP, RP11-710A11.3
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
dG9 (delta G9)
PerCP-eFluor 710
PRF1
Conjugated
PRF1
IgG2b, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
5551
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