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Perforin, eFluor 450, clone: dG9 (delta G9), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-9994-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15589856

  • £77.09 / 25 tests


Estimated Shipment date
from Supplier 30-05-2018
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Description

Description

Description: The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.Applications Reported: This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This dG9 (delta G9) antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resource Tab online. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Perforin
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P14222
cytolysin, FLH2, HPLH2, lymphocyte pore forming protein, lymphocyte pore-forming protein, perforin 1 (pore forming protein), perforin-1, PFN1, PFP, RP11-710A11.3
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
dG9 (delta G9)
eFluor 450
PRF1
Conjugated
PRF1
IgG2b, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
5551
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