PCNA, clone: PC10 (3F81), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9910-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The PC10 antibody recognizes the proliferating cell nuclear antigen (PCNA), a 36kDa protein, also known as polymerase delta auxiliary protein. PC10 antibody reacts with human, mouse, and rat PCNA. The peak expression of PCNA occurs during the S-phase.Applications Reported: The PC10 (a.k.a. 3F81) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.Applications Tested: The PC10 (a.k.a. 3F81) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The PC10 antibody has also been tested by immunocytochemistry of methanol-fixed MDCK cells and can be used at less than or equal to 5μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|P12004, P04961, P17918|
|Cyclin, DNA polymerase delta auxiliary protein, HGCN8729, MGC8367, polymerase delta accessory protein, Proliferating Cell Nuclear Antigen|
|Flow CytometryþImmunocytochemistryþImmunohistochemistryþImmunoprecipitationþWestern Blot|
|PC10 (a.k.a. 3F81)|
|Store at 2-8°C.|
For Research Use Only.