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PCNA, clone: PC10 (3F81), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9910-80

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15288227

  • £37.33 / 25µg


Estimated Shipment
23-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The PC10 antibody recognizes the proliferating cell nuclear antigen (PCNA), a 36kDa protein, also known as polymerase delta auxiliary protein. PC10 antibody reacts with human, mouse, and rat PCNA. The peak expression of PCNA occurs during the S-phase.Applications Reported: The PC10 (a.k.a. 3F81) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.Applications Tested: The PC10 (a.k.a. 3F81) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The PC10 antibody has also been tested by immunocytochemistry of methanol-fixed MDCK cells and can be used at less than or equal to 5μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

PCNA
PC10 (a.k.a. 3F81)
Unconjugated
Purified
Murine
25μg
Store at 2-8°C.
Monoclonal
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Proliferating Cell Nuclear Antigen
IgG2a
RUO
Primary
Rat
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