PARP1 (cleaved Asp214), clone: HC2R8, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-6667-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: This HC2R8 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85kDa and 25 kDa.The HC2R8 antibody specifically recognizes both the cleaved and non-cleaved forms of PARP.Applications Reported: This HC2R8 antibody has been reported for use in immunoblotting (WB).Applications Tested: This HC2R8 antibody has been tested by immunoblotting of staurosporine-treated Jurkat cells. This can be used at less than or equal to 0.1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C.|