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PARP1 (cleaved Asp214), PE, clone: HLNC4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-6668-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15527076

  • £106.91 / 25 tests


Estimated Shipment date
from Supplier 21-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This HLNC4 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85kDa and 25 kDa. The HLNC4 antibody specifically recognizes the 85kDa PARP1 fragment produced after cleavage and does not recognize the full-length 116kDa protein.The following peptide was used as the immunogen: NH2-GVDEVAKKKSKKEKDC-COOH.Applications Reported: This HLNC4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This HLNC4 antibody has been pre-titrated and tested by flow cytometric analysis of staurosporine-stimulated Jurkat cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. (Refer to Protocol B: One step protocol for intracellular (nuclear) proteins). This can be used at 5 μL (0.004 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

PARP1 (Cleaved)
HLNC4
PE
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
5μL (0.004μg)/test
aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
poly ADP-ribose polymerase
IgG2b
RUO
Primary
Human
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