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PARP1 (cleaved Asp214), eFluor 450, clone: HLNC4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-6668-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15509796

  • £195.47 / 100 tests


Estimated Shipment date
from Supplier 20-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This HLNC4 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85kDa and 25 kDa. The HLNC4 antibody specifically recognizes the 85kDa PARP1 fragment produced after cleavage and does not recognize the full-length 116kDa protein.The following peptide was used as the immunogen: NH2-GVDEVAKKKSKKEKDC-COOH.Applications Reported: This HLNC4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This HLNC4 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

PARP
HLNC4
eFluor 450
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
5μL (0.06μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
poly ADP-ribose polymerase
IgG1
RUO
Primary
Human
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