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Pan Cytokeratin, eFluor 660, clone: AE1/AE3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9003-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15570057

  • £240.50 / 100µg


EU Stock 1
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Disclaimers:

For Research Use Only.


Description

Description

Description: The monoclonal antibodies AE1 and AE3 recognize many of the acidic and basic cytokeratin family members. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these proteins and their expression can help identify the origin of a neoplasm.The AE3 monoclonal antibody recognizes the 65 to 67 triplet, 64, 59, 58, 56, 54 and 52kD proteins also known as cytokeratin 1, 2, 3, 4, 5, 6, 7, and 8 while the AE1 antibody recognizes 56.5, 54', 50, 50', 48, and 40kDa proteins (also known as CK10, 14, 15, 16 and 19). These antibodies can be used on a wide array of tissue samples from mouse, human, rat, primates (cynomolgus and rhesus), dog, cat, rabbit, and chicken.Applications Reported: This AE1/AE3 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This AE1/AE3 antibody has been tested by immunocytochemistry on methanol-fixed MCF7 cells and can be used at less than or equal to 5μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Pan Cytokeratin (AE1/AE3)
AE1/AE3
eFluor 660
Conjugated
Murine
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Immunohistochemistry (Frozen)
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
keratin, basic, acidic
IgG1
RUO
Primary
Rhesus
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