Thermo Scientific™ PageRuler™ Plus Prestained 10-250kDa Protein Ladder

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Overview
£93.60 valid until 31/12/2016
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Brand: Thermo Scientific™

Manufacturer Part Number: 26619

X2 PageRuler(TM) Plus Prestained Protein

UNSPSC: 41105337

Code: PN

Additional Details:
Additional Details: Weight: 1.36080kg



Product Code. 11832124

Quantity Price
1 £ 110.0 / Pack of 2
EU Stock 615
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Description and Specification

Specification

Description PageRuler Plus Prestained Protein Ladder, 10 to 250kDa
Detection Visible
Formulation 9 proteins from 10 to 250kDa in reducing sample buffer
Quantity 2 × 250mL
Separation Range Broad
Stained Prestained
Sufficient For 50 to 100 mini gel lanes at 5 to 10μL per lane

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70kDa and 25kDa that are colored with an orange dye and one green reference band of 10kDa. PageRuler Plus Prestained Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required before loading onto a gel.

Highlights:

  • Size range – nine proteins spanning 10 to 250kDa

  • Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

  • Sharp bands – color-coded bands of similar intesity for easy visualization

  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

  • Bright reference bands – orange at 70 and 25kDa, and green at 10kDa

  • Membrane-compatible – colored bands transfer to membranes for Western blotting

Includes:

Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.

Recommended for:

Monitoring protein migration during SDS-polyacrylamide gel electrophoresis; Monitoring protein transfer onto membranes after Western blotting; Sizing of proteins on SDS-polyacrylamide gels and Western blots