P-cadherin, clone: 12H6, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9873-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody 12H6 recognizes human P-cadherin (placental-cadherin), a calcium dependent cell-cell adhesion protein. This 118kDa transmembrane protein was originally named due to its expression in mouse placental tissue. Unfortunately, it is not expressed in human placental tissue. The function of the molecule will depend on the associations with the specific member of the cytoplasmic catenin family (alpha, beta and gamma). The specific interaction defines the strength and signaling of the cell-cell interaction. Expression is less broad than E-cadherin; P-cadherin is restricted to the basal proliferative cell layer of stratified epithelia. During development expression is found in the proliferating tissues such as hair follicle keratinocytes and the growth regions in ductal mammary tissue. There is evidence that P-cadherin can be secreted from epithelial cells during the late stages of pregnancy. P-cadherin expression is associated with poor prognosis for breast cancer while reduced expression in melanomas and squamous cell carcinomas may also result in poor prognosis.Applications Reported: This 12H6 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections (IHC-P), and immunocytochemical staining (ICC).Applications Tested: This 12H6 antibody has been tested immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human skin with low pH antigen retrieval. This antibody can be used at less than or equal to 10 μg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C.|