OVA257-264 (SIINFEKL) peptide bound to H-2Kb, PerCP-eFluor™ 710, clone: eBio25-D1.16 (25-D1.16), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 46-5743-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL peptide-pulsed mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Cells can be pulsed with the SIINFEKL peptide according to the following protocol:1. With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM.2. Incubate cells at 37°C for 2 hours.3. Wash cells with flow staining buffer.4. Proceed with cell surface staining as normal.For additional information see the references listed below.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|H-2Kb-SIINFEKL, OVA-Kb, OVA257-264 H-2Kb, OVA257-264|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.