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OVA257-264 (SIINFEKL) peptide bound to H-2Kb, Biotin, clone: eBio25-D1.16 (25-D1.16), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-5743-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15299839

  • £174.50 / 100µg


Estimated Shipment date
from Supplier 01-06-2018
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Description

Description

Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Cells can be pulsed with the SIINFEKL peptide according to the following protocol:1. With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM.2. Incubate cells at 37°C for 2 hours.3. Wash cells with flow staining buffer.4. Proceed with cell surface staining as normal.For additional information see the references listed below.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

OVA257-264 (SIINFEKL)
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
H-2Kb-SIINFEKL, OVA-Kb, OVA257-264 H-2Kb, OVA257-264
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze.
Primary
Murine
Immunohistochemistry
eBio25-D1.16 (25-D1.16)
Biotin
Conjugated
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
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