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OVA257-264 (SIINFEKL) peptide bound to H-2Kb, APC, clone: eBio25-D1.16 (25-D1.16), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-5743-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15380740

  • £186.95 / 100µg


EU Stock 3
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For Research Use Only.


Description

Description

Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL peptide pulsed mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Cells can be pulsed with the SIINFEKL peptide according to the following protocol:1. With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM.2. Incubate cells at 37°C for 2 hours.3. Wash cells with flow staining buffer.4. Proceed with cell surface staining as normal.For additional information see the references listed below.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

OVA257-264 (SIINFEKL)
eBio25-D1.16 (25-D1.16)
APC
Conjugated
Murine
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
H-2Kb-SIINFEKL, OVA-Kb
IgG1
RUO
Primary
Murine
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