OPTIZYME™ SmaI, Fisher BioReagents™
Brand: Fisher Bioreagents BP8011-5
Code : 32
Additional Details : Weight : 0.01000kg
5'...C C C^G G G...3'
3'...G G G^C C C...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 30°C
- Buffer 1: 50 - 100%
- Buffer 2: 0 - 20%
- Buffer 3: 0 - 20%
- Buffer 4: 100%
- Buffer 5: 0 - 20%
- 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- Incubation at 37°C results in 50% activity. SmaI has a half-life of 15 min at 37°C.
- Incubation at 25°C results in 100% activity.
- SmaI needs K+ to work for activity.
Dam: Never overlaps - no effect
Dcm: Never overlaps - no effect
CpG: Completely overlaps - blocked
|10X OPTIZYME™ Buffer 4|
|>90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI|
|10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol|