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OPTIZYME™ PasI, Fisher BioReagents™
Brand: Fisher Bioreagents BP8073-1
Code : 32
Additional Details : Weight : 0.01000kg
Description
5'...C C^C W G G G...3'
3'...G G G W C^C C...5'
Supplied with: 10X OPTIZYME Buffer AarI, ScaI, PasI
Conditions for 100% Activity:
- 1X OPTIZYME Buffer AarI, ScaI, PasI: 10mM Bis-Tris Propane-HCl (pH 6.5 at 37°C), 10mM MgCl2 , 100mM KCl and 0.1mg/ml BSA
- Incubate at 55°C
- Buffer 1: No Reaction
- Buffer 2: No Reaction
- Buffer 3: No Reaction
- Buffer 4: No Reaction
- Buffer 5: No Reaction
- 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- Incubation at 37°C results in 30% activity.
- Greater than 10-fold over-digestion with PasI results in star activity
Methylation Effects:
Dam: Never overlaps - no effect
Dcm: Completely overlaps - no effect
- CCm5CWGGG
Specifications
10 U/μL | |
37°C | |
>90% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with PasI | |
Keep container tightly closed | |
CC.CWGGG | |
Liquid |
>50% Glycerin, 25 to 50% Water | |
7.4 | |
10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
200 U | |
PasI |