OPTIZYME™ NotI, Fisher BioReagents™

Brand: Fisher Bioreagents

Manufacturer Part Number: BP8004-5

Optizyme NOT I restriction enzyme

UNSPSC: 12161500

Code: 32

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 11944842

Quantity Price
1 £75.74 / Each
Estimated Shipment date
from Supplier 22-11-2016
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Description and Specification


Product Name NotI
Format Liquid
Concentration 10U/μL
pH 7.4
Includes 25 to 50% Water, >50% Glycerin
Quantity 1000U
Units 1,000U
For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
Storage Buffer 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
Storage Requirements Keep container tightly closed

5'...G C^G G C C G C...3'
3'...C G C C G G^C G...5'

Conditions for 100% Activity

  • 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 , 100mM NaCl and 0.1mg/ml BSA.
  • Incubate at 37°C.
Supplied with: 10X OPTIZYME Buffer 3
Enzyme Activity in OPTIZYME buffers
  • Buffer 1: 0 - 20%
  • Buffer 2: 20 - 50%
  • Buffer 3: 100%
  • Buffer 4: 0 - 20%
  • Buffer 5: 20 - 50%
Storage Buffer:
20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with NotI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of NotI is required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Compatible Ends: Bsp120I, CfrI, Eco52I.
Methylation Effects:
Dam: never overlaps - no effect.
Dcm: never overlaps - no effect.
CpG: completely overlaps - blocked.
  • (Cleavage blocked)
  • EcoKI: never overlaps - no effect.
  • EcoBI: never overlaps - no effect.
  • Documentation