missing translation for 'onlineSavingsMsg'
Learn More

OPTIZYME™ NotI, Fisher BioReagents™

Brand:  Fisher Bioreagents BP8004-1

Code : 32

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 11934842

  • £32.80 / Each


EU Stock 4
Add to basket

Description

Description

5'...G C^G G C C G C...3'
3'...C G C C G G^C G...5'

Conditions for 100% Activity

  • 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 , 100mM NaCl and 0.1mg/ml BSA.
  • Incubate at 37°C.
Supplied with: 10X OPTIZYME Buffer 3
Enzyme Activity in OPTIZYME buffers
  • Buffer 1: 0 - 20%
  • Buffer 2: 20 - 50%
  • Buffer 3: 100%
  • Buffer 4: 0 - 20%
  • Buffer 5: 20 - 50%
Storage Buffer:
20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with NotI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of NotI is required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Compatible Ends: Bsp120I, CfrI, Eco52I.
Methylation Effects:
Dam: never overlaps - no effect.
Dcm: never overlaps - no effect.
CpG: completely overlaps - blocked.
  • GCGGCCGC
  • (Cleavage blocked)
  • EcoKI: never overlaps - no effect.
  • EcoBI: never overlaps - no effect.
  • Specifications

    Specifications

    NotI
    10U/μL
    25 to 50% Water, >50% Glycerin
    300U
    20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
    Liquid
    GC.GGCCGC
    7.4
    >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
    Keep container tightly closed
    Videos
    SDS Documentation