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OPTIZYME™ HaeIII (BsuRI), Fisher BioReagents™

Brand:  Fisher Bioreagents BP8002-5

Code : 32

Additional Details : Weight : 0.01000kg

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Product Code. 11914842

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance
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Description

Description

5'...G G^C C...3'
3'...C C^G G...5'

Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2 , 100mM KCl and 0.1mg/ml BSA.
  • Incubate at 37°C.
Supplied with: 10X OPTIZYME Buffer 5
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 – 20%
  • Buffer 2: 0 - 20%
  • Buffer 3: 0 - 20%
  • Buffer 4: 50 - 100%
  • Buffer 5: 100%
Storage Buffer:
10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with HaeIII.
Methylation Effects:
Dam: never overlaps - no effect.
Dcm: may overlap - no effect.
CpG: may overlap - no effect.
EcoKI: never overlaps - no effect.
EcoBI: never overlaps - no effect.

Specifications

Specifications

HaeIII (BsuRI)
10U/μL
25 to 50% Water, >50% Glycerin
10,000U
10mM Tris HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Liquid
GG.CC
37°C
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
Keep container tightly closed
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