OPTIZYME™ HaeIII (BsuRI), Fisher BioReagents™


Overview
Brand: Fisher Bioreagents

Manufacturer Part Number: BP8002-5

Optizyme HAE III restriction enzyme

UNSPSC: 12161500

Code: 32

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 11914842

Quantity Price
1 £ 100.2 / Each
EU Stock 2
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Description and Specification

Specification

Product Name HaeIII (BsuRI)
Format Liquid
Concentration 10U/μL
Cut Site GG.CC
Incubator Temperature 37°C
Includes 25 to 50% Water, >50% Glycerin
Quantity 10000U
Units 10,000U
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
Storage Buffer 10mM Tris HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Storage Requirements Keep container tightly closed

5'...G G^C C...3'
3'...C C^G G...5'

Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2 , 100mM KCl and 0.1mg/ml BSA.
  • Incubate at 37°C.
Supplied with: 10X OPTIZYME Buffer 5
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 – 20%
  • Buffer 2: 0 - 20%
  • Buffer 3: 0 - 20%
  • Buffer 4: 50 - 100%
  • Buffer 5: 100%
Storage Buffer:
10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with HaeIII.
Methylation Effects:
Dam: never overlaps - no effect.
Dcm: may overlap - no effect.
CpG: may overlap - no effect.
EcoKI: never overlaps - no effect.
EcoBI: never overlaps - no effect.

Documentation