OPTIZYME™ HaeIII (BsuRI), Fisher BioReagents™
Brand: Fisher Bioreagents BP8002-5
Code : 32
Additional Details : Weight : 0.01000kg
5'...G G^C C...3'
3'...C C^G G...5'
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2 , 100mM KCl and 0.1mg/ml BSA.
- Incubate at 37°C.
Enzyme Activity in OPTIZYME buffers:
- Buffer 1: 0 – 20%
- Buffer 2: 0 - 20%
- Buffer 3: 0 - 20%
- Buffer 4: 50 - 100%
- Buffer 5: 100%
10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with HaeIII.
Dam: never overlaps - no effect.
Dcm: may overlap - no effect.
CpG: may overlap - no effect.
EcoKI: never overlaps - no effect.
EcoBI: never overlaps - no effect.
|25 to 50% Water, >50% Glycerin|
|10mM Tris HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol|
|>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII|
|Keep container tightly closed|