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OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™

Brand:  Fisher Bioreagents BP8060-5

Code : 32

Additional Details : Weight : 0.01000kg

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Product Code. 11904822

  • £59.20 / Each


Estimated Shipment
06-07-2018
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Description

Description

5'...C^T N A G...3'
3'...G A N T^C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
  • Enzyme Activity in OPTIZYME buffers:
    • Buffer 1: 20 - 50%
    • Buffer 2: 20 - 50%
    • Buffer 3: 20 - 50%
    • Buffer 4: 100%
    • Buffer 5: 20 - 50%

    Storage Buffer:
    • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycero
    l
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI.
  • Compatible Ends: BbvCI, Bpu10I, Bpu1102I, Eco81I
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - effect not determined
Specifications

Specifications

DdeI (HpyF31)
10U/μL
25 to 50% Water, >50% Glycerin
7.4
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI
Keep container tightly closed
Liquid
C.TNAG
37°C
1000U
10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
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