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OCT3/4, eFluor 660, clone: EM92, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-5841-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15361930

  • £83.79 / 25µg


Estimated Shipment date
from Supplier 19-11-2018
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Description

Description

Description: The EM92 monoclonal antibody reacts with mouse and human Oct3/4, encoded by the Pou5F1 gene. Oct3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) and induced pluripotent stem (iPS) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).Applications Reported: This EM92 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This EM92 antibody has been tested by intracellular staining and flow cytometric analysis of the F9 cell line using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

OCT3/4
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q01860, P20263
Octamer-binding transcription factor 3 antibody, Oct-3, OCT3, POU domain class 5 transcription factor 1 antibody, Oct-4, OTF3 antibody.
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Murine
Flow Cytometry
EM92
eFluor 660
POU5F1
Liquid
POU5F1
IgG2a, kappa, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
18999, 5460
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